Hypo-osmotic Swelling test

Sperm vitality test

The hypo-osmotic swelling test is a sperm vitality test based on the principle that live spermatozoa swell due to the absorption of a hypo-osmotic solution.
The HOS test is based on the test procedure described in the WHO laboratory manual for the examination of human semen and sperm-cervical mucus interaction (1999).

The HOS test has a shelf life of 12 months from date of produce.

The hypo-osmotic swelling test has been CE marked according to the specifications set forth in the European Medical Device Directive 98/79/EC.

We recommend to watch our demonstration video of the HOS Test:


Europe: CE-marked - Canada: Health Canada License
Brazil: registered - Australia: registered

Product order codes

HOST Hypo-osmotic Swelling test - 5x 20ml


     Micinski P., Pawlicki K., Wielgus E., Bochenek M., Gogol P., Ficek B., Total reactive antioxidant potential and DNA fragmentation index as fertility sperm parameters, Reproductive Biology (2011),Vol.11,No.2,pp.135-44

Background information

     Drevius L.O., Eriksson H. , Osmotic swelling of mammalian spermatozoa, Exp Cell Res (1966),Vol.42,No.1,pp.136-156

     Jeyendran R.S., Van der Ven H.H., Perez-Pelaez M., Crabo B.G., Zaneveld L.J. , Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics, J Reprod Fertil (1984),Vol.70,No.1,pp.219-228


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