Spermac Stain™

Sperm morphology stain

Assessment of sperm morphology is a standard procedure in semen analysis. The Spermac Stain™ method is simple yet perfectly adequate for the detailed investigation of morphology of all parts of the spermatozoa.
The stained smears are assessed under direct bright light and oil immersion (1000x).

We recommend to watch our demonstration video of the Spermac Stain kit:


Europe: CE-marked - USA: registered - Canada: registered
Brazil: registered - Australia: registered

Product order codes

SPS050 Spermac Stain - 4 x 50mL
SPS250 Spermac Stain - 4 x 250mL

Interpretation of the results

Pictures from Professor Philip Chan (Loma Linda Univ. Gyn/Ob-IVF, USA)


     Önel T., Ayla S., Keskin I., Parlayan C., Yiğitbaşi T., Kolbaşi B., Yelke T.V., Ustabaş T.S., Leptin in sperm analysis can be a new indicator, Acta Histochemica (2019),Vol.121,pp.43-49

     Afsin M., Otludil B., Dede O., Akkus M., An examination on composition of spermatozoa obtained from pre-operative and post-operative varicocele patients, Reproductive Biology (2018),Vol.18,pp.361-367

     Bencharif D., Amirat, L., Pascal O., Anton M., Schmitt E., Desherces S., Delhomme G., Langlois M.L., Barriere P., Larrat M., Tainturier D. , The advantages of combining low-density lipoproteins with glutamine for cryopreservation of canine semen., Reprod Domest Anim (2010),Vol.45,No.2,pp.189-200

     Biricik A., Guney I., Berkil H., Benkhalifa M., Kahraman S., A male (15;15) Robertsonian translocation case with 11 previous consecutive recurrent spontaneous abortions, Marmara Medical Journal (2004),Vol.17,No.1,pp.35-38

     Cipak A., Stanic P., Duric K., Serdar T., Suchanek E. , Sperm morphology assessment according to WHO and strict criteria: method comparison and intra-laboratory variability., Biochemica Medica (2009),Vol.19,No.1,pp.87-94

     El-Harouny M.A., Zalata A.A., Naser M.E., Abo El-Atta H.M., El-Shawaf I.M., Mostafa T., Long-term ofloxacin testicular toxicity: an experimental study., Andrologia (2010),Vol.42,No.2,pp.92-96

     Levitas E., Lunenfeld E., Weisz N., Friger M. Har-Vardi I., Potashnik G., Relationship between sexual abstinence duration and the acrosome index in teratozoospermic semen: analysis of 1800 semen samples, Andrologia (2006),Vol.38,pp.110-112

     Levitas E., Lunenfeld E., Weisz N., Friger M., Potashnik G., Relationship between age and semen parameters in men with normal sperm concentration: analysis of 6022 semen samples, Andrologia (2007),Vol.39,pp.45-50

     Lockyear K.M., Goodrowe K.L., Waddell W.T. and MacDonald S.E. , Comparison of different osmolalities and egg-yolk composition in processing media for the cryopreservation of red wolf (Canis rufus) sperm., Theriogenology (2009),Vol.71,No.3,pp.469-479

     Lukanov T.H., Lichev D.I., Konova E.I., Emin A.I., Ayvazova N.P., Velkova A.V., Roussev R.G. , Flow cytometric measurement of sperm nuclear DNA fragmentation in infertile men with normal standard sperm parameters., Journal of Mens Health (2009),Vol.6,No.1,pp.50-55

     Miller J.E., Smith T.T., The effect of intracytoplasmic sperm injection and semen parameters on blastocyst development in vitro, Human Reproduction (2001),Vol.16,No.5,pp.918-924

     Parapanov RN, Nusslé S., Crausaz M., Senn A., Hausser J., Vogel P., Testis size, sperm characteristics and testosterone concentrations in four species of shrews (Mammalia, Soricidae), Animal Reproduction Science (2009),Vol.114,pp.269-278

     Rota A., Magelli C., Panzani D., Camillo F., Effect of extender, centrifugation and removal of seminal plasma on cooled-preserved Amiata donkey spermatozoa, Theriogenology (2008),Vol.69,pp.176-185

     Rota A., Milani C., Romagnoli S., Effect of post-thaw dilution with autologous prostatic fluid on dog semen motility and sperm acrosome status, Theriogenology (2007),Vol.67,pp.520-525

     Salumets A., Effects of embryological parameters on the success of fresh and frozen embryo transfers, pHD dissertation. University of Helsinki, Tartu University Press, Finland (2003),Vol.1

     Soygur B., Celik S., Celik-Ozenci C., Sati L., Effect of erythrocyte-sperm seperation medium on nuclear, acrosomal, and membrane maturity parameters in human sperm, J Assist Reprod Genet (2018),Vol.35,pp.491-501

     Svalander P., Jakobsson A-H., Forsberg A-S., Bengtsson A-C., Wikland M., The outcome of intracytoplasmic sperm injection is unrelated to 'strict criteria' sperm morphology, Human Reproduction (1996),Vol.11,No.5,pp.1019-1022

     Webb G.W., Dean M.M. , Effect of Centrifugation Technique on Post-storage Characteristics of Stallion Spermatozoa., Journal of Equine Veterinary Science (2009),Vol.29,No.9,pp.675-680

     Yakin K., Balaban B., Urman B., Is there a possible correlation between chromosomal variants and spermatogenesis?, International Journal of Urology (2005),Vol.12,pp.984-989

     Zalata A., Elhanbly S., Abdalla H., Serria M.S., Aziz A., El-Dakrooy S.A., El-Bakary A.A., Mostafa T. , In vitro study of cypermethrin on human spermatozoa and the possible protective role of vitamins C and E., Andrologia (2014),Vol.46,No.10,pp.1141-1147

     Zalata A.A., Hassan A.H., Nada H.A., Bragais F.M., Agarwal A., Mostafa T. , Follicle-stimulating hormone receptor polymorphism and seminal anti-Mullerian hormone in fertile and infertile men., Andrologia (2008),Vol.40,No.6,pp.392-397

     Zalata A.A., Morsy, H.K., Badawy Ael N., Elhanbly S., Mostafa T. , ACE gene insertion/deletion polymorphism seminal associations in infertile men., J Urol (2012),Vol.187,No.5,pp.1776-1780

Background information

     Menkveld R. , Clinical significance of the low normal sperm morphology value as proposed in the fifth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen, Asian J Androl. (2010),Vol.12,No.1,pp.47-58

     Menkveld R., Stander F.S., Kotze T.J., Kruger T.F., van Zyl J.A., The evaluation of morphological characteristics of human spermatozoa according to stricter criteria, Hum Reprod. (1990),Vol.5,No.5,pp.586-592

     Oettlé E., An improved staining technique which facilitates sequential monitoring of the acrosome state (abstract), (1986),Vol.28,No.96 (Suppl 1)

     Oettlé E., Using a new acrosome stain to evaluate sperm morphology, Vet Med. (1986),Vol.81,No.3,pp.263-6


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