Vitality stain

The eosin-nigrosin staining technique is based on the principle that dead cells will take up the eosin and as a result stain red. The nigrosin provides a dark background which makes it easier to assess the slides. The VitalScreen™ is based on the test procedure described in the WHO laboratory manual (2010).

VitalScreen has a 24 month shelf life from date of produce.

VitalScreen™ has been CE marked according to the specifications set forth in the European Medical Device Directive 98/79/EC.

We recommend to watch our demonstration video of the VitalScreen kit:


Europe: CE-marked - USA: registered - Canada: registered
Brazil: registered - Australia: registered

Product order codes

VITAL VitalScreen - 200 tests

Interpretation of the results


     Jozkow, P., M. Medras, F. Lwow, A. Zagrodna and M. Slowinska-Lisowska , Associations between physical activity and semen quality in young healthy men., Fertil Steril (2017),Vol.107,No.2,pp.373-378

     Ksiazek, A., A. Zagrodna, M. Slowinska-Lisowska, P. Jozkow, F. Lwow and M. Medras, Diet and semen quality in healthy males from the population of the lower Silesia region in Poland (The AndroLS Study)., Journal of Men's Health (2018),Vol.14,No.1,pp.e24-e31

     Laforenza, U., G. Pellavio, A. L. Marchetti, C. Omes, F. Todaro and G. Gastaldi , Aquaporin-Mediated Water and Hydrogen Peroxide Transport Is Involved in Normal Human Spermatozoa Functioning., International Journal of Molecular Sciences (2017),Vol.18,No.1,pp.66

     Lu, X., Y. Huang, H. Zhang and J. Zhao , Effect of diabetes mellitus on the quality and cytokine content of human semen., J Reprod Immunol (2017),Vol.123,pp.1-2

     Lusignan, M. F., X. Li, B. Herrero, G. Delbes and P.T.K. Chan, Effects of different cryopreservation methods on DNA integrity and sperm chromatin quality in men., Andrology (2018),Vol.6,No.6,pp.829-835

     Medras, M., F. Lwow, P. Jozkow, L. Szmigiero, A. Zagrodna, E. Zagocka and M. Slowinska-Lisowska, The quality of semen among a sample of young, healthy men from Lower Silesia (AndroLS)., Endokrynol Pol. (2017),Vol.0

     Qian, L, Decreased interleukin-11 levels in the semen of infertile males., Cytokine (2018),Vol.108,pp.57-59

     Ramzan, M. H., M. Ramzan, M. M. Khan, F. Ramzan, F. Wahab, M. A. Khan, M. Jillani and M. Shah, Human semen quality and sperm DNA damage assessed by comet assay in clinical groups., Turk J Med Sci (2015),Vol.45,No.3,pp.729-737

     Skott, M., H. Schroder, J. Hindkjaer and H.J. Kirkeby, Sperm preservation by electroejaculation before anticancer therapy., Scandinavian journal of urology (2018),Vol.17,pp.1-3

     Vaamonde, D., M. E. Da Silva-Grigoletto, J. M. Garcia-Manso, R. Vaamonde-Lemos, R. J. Swanson and S. C. Oehninger , Response of semen parameters to three training modalities., Fertil Steril (2009),Vol.92,No.6,pp.1941-1946

     Weissgerber, P., U. Kriebs, V. Tsvilovskyy, J. Olausson, O. Kretz, C. Stoerger, R. Vennekens, U. Wissenbach, R. Middendorff, V. Flockerzi and M. Freichel , Male fertility depends on Ca(2)+ absorption by TRPV6 in epididymal epithelia., Sci Signal (2011),Vol.4,No.171,pp.ra27

Background information

     Bjorndahl L., Soderlund I., Johansson S., Mohammadieh M., Pourian M.R., Kvist U., Why the WHO recommendations for eosin-nigrosin staining techniques for human sperm vitality assessment must change, J Androl (2004),Vol.25,No.5,pp.671-678

     Bjorndahl L., Soderlund I., Kvist U., Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessment, Hum Reprod (2003),Vol.18,No.4,pp.813-816


Click on the links below for more information.